bandeau nanobio
bandeau nanobio



Reflection Interference Contrast Microscopy (RICM) is a non-fluorescent technique useful to pre-investigate cellular adhesion and migration. RICM picture results from the interference between the light reflected from the glass/water interface and the cell membrane. To better detect light reflected from the membrane, circularly polarization is implemented based on the use of a specific oil immersion objective (Zeiss antiflex including a quarter wave plate on the top). RICM contrast is directly related to the height of the membrane and the local refractive index of the cytoplasm. Thus, focal adhesion plaques and stress fibers can be easily observed without any fluorescent labelling.

 


RICM_fig2
RICM_fig2

MDCKII cell on fibronectin
 
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   NEF nanoscopy
   vaTIRF nanoscopy
   nanoFCS
   RICM
   Cell patterning
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Date of update 20 décembre 2019