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- Light, nanomaterials, nanotechnologies (L2n - CNRS-EMR 7004)
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Biophotonics and Nanosensors
Topic coordinator: Rodolphe Jaffiol
Summary
Since 2006, we have been developing techniques based on fluorescence spectroscopy and imaging to study living cells. Our ambition is to propose different tools, allowing quantitative analysis at single cell level. Single molecule spectroscopy such as FCS (Fluorescence Correlation Spectroscopy) in diffracted limited and in sub-diffracted volume is employed to probe diffusion process at the nanoscale on cell membrane. Fluorescence nanoscopy, such as variable-angle Total Internal Reflection Fluorescence (vaTIRF) and Non-radiative Excitation Fluorescence (NEF), are used to investigate membrane/substrate interactions and cell adhesion.Keywords: Single molecule, Fluorescence Correlation Spectroscopy (FCS), fluorescence nano-imaging, super-resolution, Förster Resonant Energy Transfer (FRET), Total Internal Reflection Fluorescence (TIRF), Cell adhesion and migration, membrane fluidity and organization.
Research highlights by keywords
- Single nano-object spectroscopy
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Accordion content
- Nano-photochemistry and energy transfer
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- Plasmonics
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Pictured are experimental images of electromagnetic resonances in an optical antenna based on a fractal-like design, the Cayley tree. The bottom image shows the topography of the structure as seen in scanning transmission electron microscopy. This simple iterative design, combined with the use of aluminum as the antenna material, yields a broadband operating range for the antenna, spanning energies from thermal radiation up to ultraviolet. The spatial distribution of the electromagnetic resonances inside the antenna is directly imaged using electron-energy spectroscopy (EELS), a powerful technique yielding nanoscale resolution. EELS maps of four different resonances are pictured, with increasing energies from top to bottom. Each image maps a 900 nm times 900 nm area.
Aluminum Cayley trees as scalable, broadband, multiresonant optical antennas, T. Simon, X. Li, J. Martin, D. Khlopin, O. Stéfan, M. Kociak, D. Gérard, Proc. Natl. Acad. Sci. U.S.A. 119, e2116833119 (2022) - Optical forces
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Accordion content
Current research projects and related applications
Non-exhaustive list in alphabetic order- Advanced hybrid plasmonic nanosources (leading scientist: R. Bachelot)
- Chiral plasmonics (leading scientist: D. Gérard)
- Collective resonances in arrays of nanostructures (leading scientist: D. Gérard)
- Hybrid and molecular plasmonics (leading scientist: J. Proust)
- New materials for plasmonics (leading scientist: J. Plain)
- Non-linear nano-optics (leading scientists: A-L Baudrion & P-M Adam)
- Plasmon-based nanophotochemistry (leading scientist: R. Bachelot)
- Polymer nanomaterials: functionalization, micro/nano photostructuring (leading scientist: S. Jradi)
- Active Plasmonics (leading scientist: A-L. Baudrion)
- Surface enhanced Raman scattering (leading scientists: S. Jradi and P.-M. Adam)
- UV plasmonics (leading scientist: J. Martin)